In vitro 3D cell culture models have emerged as a bridge between traditional 2D cell culture models and in vivo animal models. A carefully designed 3D model provides more physiologically relevant data for cell proliferation, morphology, does response to drugs/toxins and gene expression profiles. This is because biological mechanisms that occur in #d differ significantly from 2D culture in ways more consistent with in vivo observations [1-3]. The topographically complex 3D microenvironment accurately reflects in vivo conditions than a hard & flat plastic surface. Cell adhesion, cellular structure, effector transport and mechanotransduction are also substantially different in 3D system[4]. This enables users to design experiments that previously could not be performed in conventional 2D assays, at a fraction of the cost of in vivo animal models.
