In vitro 3D cell culture models have emerged as a bridge between traditional 2D cell culture models and in vivo animal models. A carefully designed 3D model provides more physiologically relevant data for cell proliferation, morphology, does response to drugs/toxins and gene expression profiles. This is because biological mechanisms that occur in #d differ significantly from 2D culture in ways more consistent with in vivo observations [1-3]. The topographically complex 3D microenvironment accurately reflects in vivo conditions than a hard & flat plastic surface. Cell adhesion, cellular structure, effector transport and mechanotransduction are also substantially different in 3D system. This enables users to design experiments that previously could not be performed in conventional 2D assays, at a fraction of the cost of in vivo animal models.